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Texas Children's Hospital and Baylor College of Medicine

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

¹Center for Human Immunobiology, Texas Children's Hospital and Baylor College of Medicine

Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.