Shriners Burns Hospital
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
Caroline N. Jones *1,2,3, Anh N. Hoang *1,2,3, Laurie Dimisko 1, Bashar Hamza 1, Joseph Martel 1,4, Daniel Irimia 1,2,3
1The BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital, 2Harvard Medical School, 3Shriners Burns Hospital, 4Harvard University School of Engineering and Applied Sciences
This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.
Developmental Biology
Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
Felix Ellett 1, Daniel Irimia 1
1BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital–Harvard Medical School–Shriners Burns Hospital
Microinjection of zebrafish embryos and larvae is a crucial but challenging technique used in many zebrafish models. Here, we present a range of microscale tools to aid in the stabilization and orientation of zebrafish for both microinjection and imaging.
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