Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung PreparationKathirvel Kandasamy 1, Kaushik Parthasarathi 1,2
1Department of Physiology, The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering, The University of Tennessee Health Science Center
The isolated blood-perfused lung preparation makes it feasible to visualize microvessel networks on the lung surface. Here we describe an approach to quantify permeability of single microvessels in isolated lungs using real time fluorescence imaging.
