Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor TechnologyJon A. Weidanz 1, Krysten L. Doll 1, Soumya Mohana-Sundaram 1, Timea Wichner 1, Devin B. Lowe 1, Susanne Gimlin 2, Debra Wawro Weidanz 2, Robert Magnusson 2,3, Oriana E. Hawkins 1
1Experimmune, A Center for Immunotherapeutic Development, Department of Immunotherapeutics and Biotechnology, Texas Tech University Health Sciences Center, 2Resonant Sensors Incorporated, 3University of Texas Arlington
Intact class I HLA/peptide complexes are shed by cancer cells, representing a potential relevant cancer biomarker. Utilizing label-free sensor technology and T-cell receptor mimicking monoclonal antibodies, detection of shed MIF/HLA-A*02:01 complexes in MDA-MB-231 cell supernatants, spiked human serum, and patient plasma is demonstrated, enabling development of a novel cancer diagnostic platform.
