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The University of Michigan

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Derek S. Samarian¹, Nicholas S. Jakubovics², Ting L. Luo¹, Alexander H. Rickard¹

¹Department of Epidemiology, School of Public Health, The University of Michigan, ²Centre for Oral Health Research, School of Dental Sciences, Newcastle University

The goal of this methods paper is to describe the use of a microfluidic system for the development of multi-species biofilms that contain species typically identified in human supragingival dental plaque. Methods to describe biofilm architecture, biofilm viability, and an approach to harvest biofilm for culture-dependent or culture-independent analyses are highlighted.

Biology

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Zhen Wang^1,4, Ramon Ocadiz-Ruiz¹, Sinju Sundaresan¹, Lin Ding¹, Michael Hayes¹, Nirakar Sahoo³, Haoxing Xu^1,2, Juanita L. Merchant^1,2,5

¹Department of Internal Medicine-Gastroenterology, University of Michigan, ²Department of Molecular and Integrative Physiology, University of Michigan, ³Department of Molecular, Cellular and Developmental Biology, University of Michigan, ⁴Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, ⁵Division of Gastroenterology, University of Arizona College of Medicine

Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis.

Developmental Biology

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Junya Shiota¹, Nureen H. Mohamad Zaki¹, Juanita L. Merchant^1,2, Linda C. Samuelson^1,2, Nataliya Razumilava¹

¹Departments of Internal Medicine, The University of Michigan, ²Molecular & Integrative Physiology, The University of Michigan

This protocol describes the production of a mouse extrahepatic bile duct 3-dimensional organoid system. These biliary organoids can be maintained in culture to study cholangiocyte biology. Biliary organoids express markers of both progenitor and biliary cells and are composed of polarized epithelial cells.