Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular MitophagyGabriele Di Sante 1, Mathew C. Casimiro 1, Timothy G. Pestell 2, Richard G. Pestell 1,2,3
1Department of Cancer Biology, Thomas Jefferson University, SKCC, 2Department of Medical Oncology, Thomas Jefferson University, SKCC, 3Kazan Federal University
Herein, we describe in detail a time-lapse video microscopy approach to measuring the temporal recruitment of EYFP-Parkin during the selective removal of damaged mitochondria. This dynamic process of EYFP-Parkin-dependent removal of damaged mitochondria can be used as an indicator of cellular health under different experimental conditions.
