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Normandy University

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Erika S. Hue^1,2,3, Christine I. Fortier^1,3, Aurélie M. Laurent¹, Yann F. Quesnelle¹, Guillaume D. Fortier^1,2,3, Loïc J. Legrand^1,2,3, Stephane L. Pronost^1,2,3

¹LABÉO Frank Duncombe, ²Unité de Risques Microbiens (U2RM, EA 4655), Normandy University, ³Hippolia Foundation

Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.