A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron CultureZhongming Lu *1,2, Mariel Piechowicz *1, Shenfeng Qiu 1
1Department of Basic Medical Sciences, University of Arizona College of Medicine-Phoenix, 2Jiangsu Provincial Center for Disease Control and Prevention
Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.
