Iniciar sesión

Martin-Luther Hospital, Academic Teaching Institution of Charité Universitätsmedizin Berlin

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

Pauline Teuffel¹, Miriam Goebel-Stengel^1,2, Tobias Hofmann¹, Philip Prinz¹, Sophie Scharner¹, Jan L. Körner³, Carsten Grötzinger³, Matthias Rose¹, Burghard F. Klapp¹, Andreas Stengel¹

¹Charité Center for Internal Medicine and Dermatology, Division General Internal and Psychosomatic Medicine, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, ²Department of Internal Medicine, Institute of Neurogastroenterology and Motility, Martin-Luther Hospital, Academic Teaching Institution of Charité Universitätsmedizin Berlin, ³Department of Hepatology and Gastroenterology, Molecular Cancer Research Center (MKFZ), Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin

The RAPID blood processing method can be used in humans and yields higher peptide levels as well as allows for assessment of the correct molecular form. Therefore, this method will be a valuable tool in peptide research.

Medicine

Sucrose Preference and Novelty-Induced Hypophagia Tests in Rats using an Automated Food Intake Monitoring System

Martha Anna Schalla^*1, Stephanie Gladys Kühne^*1, Tiemo Friedrich¹, Vivien Hanel¹, Peter Kobelt¹, Miriam Goebel-Stengel^1,2,3, Matthias Rose^1,4, Andreas Stengel^1,3

¹Charité Center for Internal Medicine and Dermatology, Department for Psychosomatic Medicine, Charité-Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, ²Department of Internal Medicine, Helios Clinic, ³Department of Psychosomatic Medicine and Psychotherapy, University Hospital Tübingen, ⁴Department of Quantitative Health Sciences, University of Massachusetts Medical School

Presented here is a protocol to study depression-like and anhedonic behavior in rats. It combines two well-established behavioral methods, the sucrose preference and novelty-induced hypophagia tests, with an automated food and liquid intake monitoring system, to indirectly investigate rodent behavior using surrogate parameters.