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Hôpital Maisonneuve-Rosemont

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Preparation and Applications of Organotypic Thymic Slice Cultures

Aditi Sood^*1,2, Mengqi Dong^*1,2, Heather J. Melichar^1,3

¹Centre de Recherche, Hôpital Maisonneuve-Rosemont, ²Department of Microbiology, Infectiology and Immunology, Université de Montréal, ³Department of Medicine, Université de Montréal

We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.