A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick EmbryosManuel Schmitz *1, Ben K. A. Nelemans *1, Theodoor H. Smit 2
1Department of Orthopaedic Surgery, VU University Medical Center Amsterdam, MOVE Research Institute Amsterdam, 2Department of Anatomy, Embryology & Physiology, Academic Medical Center Amsterdam
Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique1 that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.
