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University of California, Irvine School of Medicine

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Elisa Romeo 1, Silvia Pontis 1, Stefano Ponzano 1, Fabiola Bonezzi 1, Marco Migliore 1, Simona Di Martino 1, Maria Summa 1, Daniele Piomelli 1,2
1Drug Discovery and Development, Istituto Italiano di Tecnologia, 2Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry, University of California, Irvine School of Medicine

Here, we describe the preparation and use of an activity-based probe (ARN14686, undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate) that allows for the detection and quantification of the active form of the proinflammatory enzyme N-acylethanolamine acid amidase (NAAA), both in vitro and ex vivo.

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