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University of California, Irvine School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Elisa Romeo¹, Silvia Pontis¹, Stefano Ponzano¹, Fabiola Bonezzi¹, Marco Migliore¹, Simona Di Martino¹, Maria Summa¹, Daniele Piomelli^1,2

¹Drug Discovery and Development, Istituto Italiano di Tecnologia, ²Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry, University of California, Irvine School of Medicine

Here, we describe the preparation and use of an activity-based probe (ARN14686, undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate) that allows for the detection and quantification of the active form of the proinflammatory enzyme N-acylethanolamine acid amidase (NAAA), both in vitro and ex vivo.