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Chang Gung Memorial Hospital, Chang Gung University College of Medicine

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

A Method for Measuring RNA N⁶-methyladenosine Modifications in Cells and Tissues

Chao-Yung Wang¹, Mei-Hsiu Lin¹, Hui-Ting Su¹

¹Department of Cardiology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine

A modified northern blotting method for measuring N⁶-methyladenosine (m⁶A) modifications in RNA is described. The current method can detect modifications in diverse RNAs and controls under various experimental designs.