Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow CytometryBenoîte Bourdin 1, Emilie Segura 1, Marie-Philippe Tétreault 1, Sylvie Lesage 2, Lucie Parent 1
1Département de Physiologie Moléculaire et Intégrative, Montreal Heart Institute Research Centre, 2Département de Microbiologie, Infectiologie, Immunologie, Centre de recherche de l'Hôpital Maisonneuve-Rosemont
Inherited cardiac arrhythmias are often caused by mutations that alter the surface delivery of one or more ion channels. Here, we adapt flow cytometry assays to provide a quantification of the relative total and cell surface protein expression of recombinant ion channels expressed in tsA-201 cells.
