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Friedrich Schiller University, Jena

2 ARTICLES PUBLISHED IN JoVE

Biology

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Anna Foerster¹, Mario Assenmacher¹, Michaela Niemoeller¹, Elly Rankin¹, Mariette Mohaupt¹, Anne Richter¹

This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.

Genetics

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Theresa Hölscher¹, Anna Dragoš¹, Ramses Gallegos-Monterrosa¹, Marivic Martin¹, Eisha Mhatre¹, Anne Richter¹, Ákos T. Kovács¹

¹Terrestrial Biofilms Group, Institute of Microbiology, Friedrich Schiller University, Jena

The role of assortment (spatial segregation) in evolutionary scenarios can be examined using simple microbial systems in the laboratory that allow controlled adjustment of spatial distribution. By modifying the founder cell density, various assortment levels can be visualized using fluorescently labelled bacterial strains in the colony biofilms of Bacillus subtilis.