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University Medical Center Giessen and Marburg

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Anna Lena Jung¹, Kerstin Hoffmann¹, Christina E. Herkt¹, Christine Schulz¹, Wilhelm Bertrams¹, Bernd Schmeck^1,2,3

¹Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, ²German Center for Lung Research, ³Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg

Here, we describe the purification of Legionella pneumophila (L. pneumophila) outer membrane vesicles (OMVs) from liquid cultures. These purified vesicles are then used for the treatment of macrophages to analyze their pro-inflammatory potential.