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Katholieke Universiteit Leuven (KU Leuven)

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

Katrien De Clercq^*1, Aurélie Hennes^*1, Joris Vriens¹

¹Department of Development and Regeneration, Katholieke Universiteit Leuven (KU Leuven)

This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.

Neuroscience

Horizontal Hippocampal Slices of the Mouse Brain

Evelien Van Hoeymissen^1,2, Koenraad Philippaert², Rudi Vennekens², Joris Vriens^*1, Katharina Held^*1,2

¹Laboratory of Endometrium, Endometriosis and Reproductive Medicine, Department of Development and Regeneration, KU Leuven, ²Laboratory of Ion Channel Research, VIB-KU Leuven Center for Brain and Disease Research, Leuven, Belgium and Department of Molecular Medicine, KU Leuven

This article aims to describe a systematic protocol to obtain horizontal hippocampal brain slices in mice. The objective of this methodology is to preserve the integrity of hippocampal fiber pathways, such as the perforant path and the mossy fiber tract to assess dentate gyrus related neurological processes.