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Stazione Zoologica, Anton Dohrn, Naples (Italy)

2 ARTICLES PUBLISHED IN JoVE

Biology

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana

Loredana Manfra¹, Federica Savorelli², Marco Pisapia¹, Erika Magaletti¹, Anna Maria Cicero¹

¹Department of Environmental Quality Monitoring, Institute for Environmental Protection and Research, ²Regional Agency for Environmental Protection in Emilia-Romagna

This study concerns the development and standardization of a valuable methodological protocol to determine long-term (14 days) lethal toxicity exerted by chemical substances, industrial wastewater or sewage and liquid environmental samples on the saltwater crustacean, Artemia franciscana.

Environment

Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants

Alice Rotini^1,2, Loredana Manfra^1,3, Filippo Spanu², Marco Pisapia¹, Anna Maria Cicero¹, Luciana Migliore²

¹Institute for Environmental Protection and Research (ISPRA), Rome (Italy), ²Department of Biology, Tor Vergata University, Rome (Italy), ³Department of Biology and Evolution of Marine Organisms, Stazione Zoologica, Anton Dohrn, Naples (Italy)

This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC₅₀, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure.