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CSIR-National Chemical Laboratory

2 ARTICLES PUBLISHED IN JoVE

Environment

Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance

Santosh G. Tupe¹, Ejaj K. Pathan¹, Mukund V. Deshpande¹

¹Biochemical Sciences Division, CSIR-National Chemical Laboratory

Here, we report the different stages involved in the knowledge-based development of an effective mycoinsecticide, including the isolation, identification, screening, and selection of the "best-fit" entomopathogenic fungus, Metarhizium anisopliae, for the control of insect pests in agriculture.

Biology

Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera

Sharada D. Mohite^1,2, Yogita P. Patil^1,2, Vikram J. Nichit^1,2, Rakesh S. Joshi^1,2

¹Biochemical Sciences Division, CSIR-National Chemical Laboratory, ²Academy of Scientific and Innovative Research (AcSIR)

This protocol describes the obligate feeding assay to evaluate the potentially toxic effect of a phytochemical on the lepidopteran insect larvae. This is a highly scalable insect bioassay, easy to optimize the sublethal and lethal dose, deterrent activity, and physiological effect. This could be used for screening eco-friendly insecticides.