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Universitat de Barcelona, L'Hospitalet de Llobregat

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Xavier Morató^*1,2, Marc López-Cano^*1,2, Paula M. Canas³, Rodrigo A. Cunha³, Francisco Ciruela^1,2

¹Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL, Universitat de Barcelona, L'Hospitalet de Llobregat, ²Institut de Neurociències, Universitat de Barcelona, ³Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra

Here, we present a brain membrane fractionation protocol that represents a robust procedure to isolate proteins belonging to different synaptic compartments.