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University Paris Descartes

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Elisa Peranzoni^*1, Houcine Bougherara^*1, Sarah Barrin^*1, Audrey Mansuet-Lupo^2,3, Marco Alifano⁴, Diane Damotte^2,3, Emmanuel Donnadieu¹

¹Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, ²Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, ³INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, ⁴Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes

This protocol describes a method to image resident tumor-infiltrating CD8 T cells labeled with fluorescently coupled antibodies within human lung tumor slices. This technique permits real-time analyses of CD8 T cell migration using confocal microscopy.

JoVE Journal

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Charles Decraene^1,2, Amanda Bortolini Silveira¹, Marc Michel¹, François-Clément Bidard^1,3,4, Jean-Yves Pierga^1,3,5, Marc-Henri Stern^1,6, Charlotte Proudhon¹

¹Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, ²CNRS UMR144, Institut Curie, PSL Research University, ³Department of Medical Oncology, Institut Curie, PSL Research University, ⁴University Versailles Saint-Quentin-en-Yvelines, ⁵University Paris Descartes, ⁶Inserm U830, Institut Curie, PSL Research University

Here, we present a protocol to accurately quantify multiple genetic alterations of a target region in a single reaction using drop-off ddPCR and a unique pair of hydrolysis probes.