Max Planck Institute of Molecular Plant Physiology, Golm, Germany
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Mohamed Salem 1,2, Michal Bernach 1, Krzysztof Bajdzienko 1, Patrick Giavalisco 1
1Max Planck Institute of Molecular Plant Physiology, Golm, Germany, 2Department of Pharmacognosy, Faculty of Pharmacy, Cairo University
A protocol for comprehensive extraction of lipids, metabolites and proteins from biological tissues using one sample is presented.
Developmental Biology
A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
Umarah Mubeen 1, Lais Albuquerque Giraldi 1, Jessica Jüppner 1, Patrick Giavalisco 1,2
1Max Planck Institute of Molecular Plant Physiology, 2Max Planck Institute of Biology of Ageing
System-wide analysis of multiple biomolecules is crucial to gain functional and mechanistic insights into biological processes. Hereby, an extensive protocol is described for high throughput extraction of lipids, metabolites, proteins and starch from a single sample harvested from synchronized Chlamydomonas culture.
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