Quantitative Immunofluorescence to Measure Global Localized TranslationJonathan Bergeman 1, Marc-Étienne Huot 1,2
1Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie, Université Laval, 2CRCHU de Québec: L’Hôtel-Dieu de Québec
This manuscript describes a method to visualize and quantify localized translation events in subcellular compartments. The approach proposed in this manuscript requires a basic confocal imaging system and reagents and is rapid and cost-effective.
