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Consejo Superior de Investigaciones Científicas (CSIC)

2 ARTICLES PUBLISHED IN JoVE

Biology

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Chrstine Bäuerl^*1, Özgun C.O. Umu^*2, Pablo E. Hernandez³, Dzung B. Diep⁴, Gaspar Pérez-Martínez¹

¹Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), ²Department of Food Safety and Infection Biology, Norwegian University of Life Sciences (NMBU), ³Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), ⁴Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)

Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model.

Environment

Automatic Image Processing to Determine the Community Size Structure of Riverine Macroinvertebrates

Rosa Gurí^1,4, Ignasi Arranz^2,4, Marc Ordeix^1,4, Carmen García-Comas^3,4

¹Center for the Study of Mediterranean Rivers (CERM), Universitat de Vic - Universitat Central de Catalunya, ²Laboratoire Evolution et Diversité Biologique (EDB), UMR5174, Université Toulouse 3 Paul Sabatier, Centre national de la recherche scientifique (CNRS), Institut de Recherche pour le Développement (IRD), ³Department of Marine Biology and Oceanography, Institut de Ciències del Mar, Consejo Superior de Investigaciones Científicas (CSIC), ⁴Aquatic Ecology Group, Universitat de Vic - Universitat Central de Catalunya

The article is based on the creation of an adapted protocol to scan, detect, sort, and identify digitized objects corresponding to benthic river macroinvertebrates using a semi-automatic imaging procedure. This procedure allows the acquisition of the individual size distributions and size metrics of a macroinvertebrate community in about 1 h.