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The Saban Research Institute of Children's Hospital Los Angeles

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Marta H. Kubala^1,2, Yves A. DeClerck^1,2,3

¹Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, ²The Saban Research Institute of Children's Hospital Los Angeles, ³Department of Biochemistry and Molecular Medicine, University of Southern California

This protocol serves as a scheme for setting up a functional Tet-ON system in cancer cell lines and its subsequent use, in particular for studying the role of tumor cell-derived proteins in recruitment of monocytes/macrophages to the tumor microenvironment.