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Institut de Cancérologie de la Loire - Lucien Neuwirth

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

Jean-Baptiste Guy^*1,2, Sophie Espenel^*1,2, Alexis Vallard^1,2, Priscillia Battiston-Montagne¹, Anne-Sophie Wozny^1,3, Dominique Ardail^1,3, Gersende Alphonse^1,3, Chloé Rancoule^1,2, Claire Rodriguez-Lafrasse^1,3, Nicolas Magne^1,2

¹UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, ²Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, ³Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud

This study reports two different methods for the analysis of cell invasion and migration: the Boyden chamber assay and the in vitro video microscope-based wound-healing assay. The protocols for these two experiments are described, and their benefits and disadvantages are compared.