Kings College London
2 ARTICLES PUBLISHED IN JoVE
JoVE Journal
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
Caroline Haupt *1, Tommy Hofmann *1, Sabine Wittig *1, Susann Kostmann 1, Argyris Politis 2, Carla Schmidt 1
1Interdisciplinary research center HALOmem, Martin Luther University Halle-Wittenberg, 2Department of Chemistry, Kings College London
The architecture of protein complexes is essential for their function. Combining various mass spectrometric techniques proved powerful to study their assembly. We provide protocols for chemical cross-linking and native mass spectrometry and show how these complementary techniques help to elucidate the architecture of multi-subunit protein assemblies.
Research
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
Zainab Ahdash 1, Andy M. Lau 1, Chloe Martens 1, Argyris Politis 1
1Department of Chemistry, King's College London
Mass spectrometry (MS) has emerged as an important tool for the investigation of structure and dynamics of macromolecular assemblies. Here, we integrate MS-based approaches to interrogate protein complex formation and ligand binding.
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