Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's MembraneHui Cai 1, Jie Gong 1, Lucian V. Del Priore 1, Tongalp H. Tezel 2, Mark A. Fields 1
1Department of Ophthalmology and Visual Science, Yale School of Medicine, 2Edward S. Harkness Eye Institute, Columbia University School of Medicine
The goal of this protocol is to demonstrate the culturing of retinal pigment epithelial (RPE) cells on aged and/or diseased human Bruch's membrane. This method is suitable to study RPE cell behavior on a compromised extracellular matrix.
