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L'insitut National des Sciences Appliquées (INSA) de Lyon

1 ARTICLES PUBLISHED IN JoVE

Chemistry

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Lukasz Bozycki¹, Magdalena Komiazyk¹, Saida Mebarek^2,3,4,5,6, Rene Buchet^2,3,4,5,6, Slawomir Pikula¹, Agnieszka Strzelecka-Kiliszek¹

¹Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, ²Université de Lyon, ³Université Lyon 1, ⁴L'insitut National des Sciences Appliquées (INSA) de Lyon, ⁵Ecole Supérieure de Chimie Physique Electronique (CPE) Lyon, ⁶Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR), L'institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS)

We present a protocol to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. Their mineralization profiles were analyzed by Alizarin Red-S (AR-S) staining, ultraviolet (UV) light visualization, transmission electron microscopy (TEM) imaging and energy dispersive X-ray microanalysis (EDX).