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National Cancer Research Center (CNIO)

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration

Maria Riedel¹, Martin F. Berthelsen¹, Latifa Bakiri², Erwin F. Wagner², Martin K. Thomsen¹

¹Department of Clinical Medicine, Aarhus University, ²GDD, Cancer Cell Biology Program, National Cancer Research Center (CNIO)

This protocol describes a newly established method for virus delivery to the murine prostate. Using either CRISPR/Cas9 technology, gene overexpression, or Cre recombinase delivery, the technique allows orthotopic alteration of gene expression and implements a novel mouse model for prostate cancer.