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Mayo Clinic Graduate School of Biomedical Sciences

2 ARTICLES PUBLISHED IN JoVE

Biology

Immunostaining of Dissected Zebrafish Embryonic Heart

¹Division of Cardiovascular Diseases, Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine

A rapid way to conduct immunostaining of zebrafish embryonic heart is described. Compared to the whole mount immunostaining approach, this method dramatically increases the penetration of the antibodies, which allows obtaining high resolution images that reveal cellular/subcellular structures in the heart within a much reduced processing time.

Medicine

A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish

Xiao Ma^*1,2,3, Yonghe Ding^*2,3, Yong Wang^2,3,4, Xiaolei Xu^1,2,3

¹Clinical and Translational Sciences Track, Mayo Clinic Graduate School of Biomedical Sciences, ²Department of Biochemistry and Molecular Biology, Mayo Clinic, ³Division of Cardiovascular Diseases, Mayo Clinic, ⁴Institute of Life Science, Beijing University of Chinese Medicine

A method to generate a doxorubicin-induced cardiomyopathy model in adult zebrafish (Danio rerio) is described here. Two alternative ways of intraperitoneal injection are presented and conditions to reduce variations among different experimental groups are discussed.