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Shanghai Jiao Tong University Affiliated Sixth People's Hospital

4 ARTICLES PUBLISHED IN JoVE

Education

A Clinical Trial Assessing the Safety, Efficacy, and Delivery of Olive-Oil-Based Three-Chamber Bags for Parenteral Nutrition

Zhenyi Jia¹, Jun Yang¹, Huanlong Qin^1,2

¹Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, ²Department of General Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University

Here, we present a protocol for a study comparing the efficacy, safety, and delivery of olive-oil-based 3CB and soybean-oil-based CoB formulations in adults requiring parenteral nutrition. The results revealed that olive-oil-based 3CBs is non-inferior and well tolerated compared to soybean formulations.

Biochemistry

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

Ping Wei^*1, Qihong Liu^*2, Wen Xue², Jiwu Wang²

¹Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, ²Department of Anatomy of Physiology, Shanghai Jiao Tong University School of Medicine

We present a protocol for a colorimetric assay of citrate synthase activity for quantification of intact mitochondrial mass in Drosophila tissue homogenates.

Developmental Biology

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Yong Wang^*1, Ding Zhang^*2, Fang Zhou², Meijun Zhou², Qiujie Li¹, Jingyu Chen³, Jun Yang^1,2

¹Department of Physiology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, ²Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, ³Wuxi Lung Transplant Center, Wuxi People's Hospital affiliated to Nanjing Medical University

Presented here is a protocol for whole-mount in situ RNA hybridization analysis in zebrafish and tube formation assay in patient-derived induced pluripotent stem cell-derived endothelial cells to study the role of endoglin in vascular formation.

Neuroscience

Evaluating Postural Control and Lower-extremity Muscle Activation in Individuals with Chronic Ankle Instability

Lulu Yin¹, Zhangqi Lai², Xiaoyue Hu², Kun Liu³, Lin Wang²

¹Department of Critical Care Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, ²School of Kinesiology, Shanghai University of Sport, ³Department of Rehabilitation Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital

Individuals with chronic ankle instability (CAI) exhibit postural control deficiency and delayed muscle activation of lower extremities. Computerized dynamic posturography combined with surface electromyography provides insights into the coordination of the visual, somatosensory, and vestibular systems with muscle activation regulation to maintain postural stability in individuals with CAI.