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Martin-Luther-Universitat Halle-Wittenberg

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Venkatasalam Shanmugabalaji¹, Véronique Douet¹, Birgit Agne², Felix Kessler¹

¹Laboratory of Plant Physiology, University of Neuchatel, ²Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.