A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer CellsBiao Xi 1, Robert Berahovich 2, Hua Zhou 2, Shirley Xu 2, Yuehua Wei 2, Jasper Guan 2, Hizkia Harto 2, Jian Guan 2, Lijun Wu 2, David Santa Ana 1,3, Fabio Cerignoil 1, Brandon Lamarche 1, Yama A. Abassi 1, Vita Golubovskaya 2
1ACEA Biosciences, Inc, 2ProMab Biotechnologies. Inc, 3ACEA Therapeutics
We describe a quantitative real-time in vitro cytolysis assay system to evaluate the potency of chimeric antigen receptor T cells targeting liquid and solid tumor cells. This protocol can be extended to assess other immune effector cells, as well as combination treatments.
