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United States Department of Agriculture (USDA), Agricultural Research Service (ARS)

1 ARTICLES PUBLISHED IN JoVE

Environment

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Nicole G. Novak¹, Frances G. Perez², Richard W. Jones², Susan D. Lawrence¹

¹Invasive Insect Biocontrol and Behavior Lab, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), ²Genetic Improvement for Fruits and Vegetables Lab, USDA-ARS

The presented method creates natural herbivore damaged plant tissue through the application of Manduca sexta larvae to detached leaves of potato. The plant tissue is assayed for expression of six transcription factor homologs involved in early responses to insect herbivory.