Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of LyssavirusesYuyang Wang *1, Weidi Xu *1,2, Huancheng Guo 1, Wenjie Gong 1, Biao He 1, Zhongzhong Tu 1, Changchun Tu 1,3, Ye Feng 1
1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.
