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Showa University School of Pharmacy

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Takashi Obama^*1, Takuro Miyazaki^*2, Toshihiro Aiuchi¹, Akira Miyazaki², Hiroyuki Itabe¹

¹Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, ²Department of Biochemistry, Showa University School of Medicine

Here, we present a protocol for an on-membrane digestion technique for the preparation of samples for mass spectrometry. This technique facilitates the convenient analysis of protein–protein interactions.