Nanosystems Initiative Munich
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
Günther Pardatscher *1, Matthaeus Schwarz-Schilling *1, Sandra Sagredo 1, Friedrich C. Simmel 1
1Physics Department, Technical University of Munich
We describe a simple lithographic procedure for the immobilization of gene-length DNA molecules on a surface, which can be used to perform cell-free gene expression experiments on biochips.
Bioengineering
In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
Kilian Vogele 1, Thomas Frank 1, Lukas Gasser 1, Marisa A. Goetzfried 1, Mathias W. Hackl 2, Stephan A. Sieber 2, Friedrich C. Simmel 1,3, Tobias Pirzer 1
1Physics of Synthetic Systems - E14, Physics-Department and ZNN, Technische Universität München, 2Departmant of Chemistry, Center for Integrated Protein Science (CIPSM), Technische Universität München, 3Nanosystems Initiative Munich
Presented here are protocols for the creation of peptide-based small unilamellar vesicles capable of growth. To facilitate in vesiculo production of the membrane peptide, these vesicles are equipped with a transcription-translation system and the peptide-encoding plasmid.
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