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Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU)

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Thomas Labadie¹, Quentin Grassin², Christophe Batéjat², Jean-Claude Manuguerra², India Leclercq^2,3

¹Department of Infection Biology, London School of Hygiene and Tropical Medicine, ²Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), ³Cellule Pasteur, Université de Paris

Reported here is a protocol for the quantification of infectious viral particles using real-time monitoring of electrical impedance of infected cells. A practical application of this method is presented by quantifying influenza A virus decay under different physicochemical parameters mimicking environmental conditions.