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Lawrence Berkeley Laboratory

2 ARTICLES PUBLISHED IN JoVE

Biology

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

¹Department of Biochemistry and Microbiology, University of Victoria

Here we present a cost-effective method for defining chemical-genetic interactions in budding yeast. The approach is built on fundamental techniques in yeast molecular biology and is well suited for the mechanistic interrogation of small to medium collections of chemicals and other media environments.

Biochemistry

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Mowei Zhou¹, Shadan H. Abdali¹, David Dilworth², Lifeng Liu², Benjamin Cole², Neha Malhan¹, Amir H. Ahkami¹, Tanya E. Winkler¹, Joy Hollingsworth³, Julie Sievert³, Jeff Dahlberg³, Robert Hutmacher^4,5, Mary Madera⁶, Judith A. Owiti⁶, Kim K. Hixson¹, Peggy G. Lemaux⁶, Christer Jansson¹, Ljiljana Paša-Tolić¹

¹Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, ²DOE-Joint Genome Institute, Lawrence Berkeley Laboratory, ³Kearney Agricultural Research and Extension Center, University of California Agriculture and Natural Resources, ⁴West Side Research and Extension Center, University of California, ⁵Department of Plant Sciences, University of California, Davis, ⁶Department of Plant and Microbial Biology, University of California, Berkeley

The protocol has been developed to effectively extract intact histones from sorghum leaf materials for profiling of histone post-translational modifications that can serve as potential epigenetic markers to aid engineering drought resistant crops.