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University Medical Center Goettingen, Goettingen, Germany

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca²⁺ Transients and Membrane Currents

Niels Voigt^*1,2, Xiao-Bo Zhou^*2, Dobromir Dobrev^1,2

¹Institute of Pharmacology, University of Duisburg-Essen , ²Division of Experimental Cardiology, University of Heidelberg

We describe the isolation of human atrial myocytes which can be used for intracellular Ca²⁺ measurements in combination with electrophysiological patch-clamp studies.

Bioengineering

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Fitzwilliam Seibertz^1,2, Martyn Reynolds³, Niels Voigt^1,2,4

¹Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, Germany, ²DZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Germany, ³Cairn Research Ltd, Faversham, United Kingdom, ⁴Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Goettingen, Germany

Here we describe optical acquisition and characterization of action potentials from induced pluripotent stem cell derived cardiomyocytes using a high-speed modular photometry system.