Iniciar sesión

City University of New York School of Medicine at City College

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Jose A. Pino^*1, Nora Awadallah^*2, Alessandra M. Norris³, Gonzalo E. Torres²

¹Department of Medicine, School of Medicine, Universidad de Atacama, ²Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, ³Department of Pharmacology and Therapeutics, University of Florida College of Medicine

This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.