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Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research)

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Lukas Pekarek¹, Stefan Buck¹, Neva Caliskan^1,2

¹Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), ²Medical Faculty, Julius-Maximilians University Würzburg

This protocol presents a complete experimental workflow for studying RNA-protein interactions using optical tweezers. Several possible experimental setups are outlined including the combination of optical tweezers with confocal microscopy.