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Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Jing Guo¹, Sifan You¹, Zhichang Wang¹, Jinbo Peng¹, Runze Ma¹, Ying Jiang^1,2

¹International Center for Quantum Materials, School of Physics, Peking University, ²Collaborative Innovation Center of Quantum Matter

Here, we present a protocol to investigate the structure and dynamics of interfacial water at the atomic scale, in terms of submolecular resolution imaging, molecular manipulation, and single-bond vibrational spectroscopy.

Bioengineering

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

Zhiwei Xie^1,2,3, Jizhou Shi⁴, Min Zong³, Qiuping Xu¹, Chang Liu¹, Jie Wen¹, Qun Zhang¹, Panpan Liu¹, Guanyi Liu¹, Jing Guo^2,5, Xunwei Wu¹

¹Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ²Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ³Department of Stomatology, Shengli Oilfield Central Hospital, ⁴Department of Pediatric Surgery, Shengli Oilfield Central Hospital, ⁵Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.