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Universidad Católica del Norte

3 ARTICLES PUBLISHED IN JoVE

Behavior

An Inescapable Cat Odor Exposure Protocol for Studying Innate and Contextual Threat Conditioning in Rats

María Rodríguez^1,2, Marco Contreras³, Marcos Domic-Siede⁴, Francisco Ceric⁵, Fernando Torrealba¹

¹Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, ²Instituto de Ciencias Sociales, Universidad de O'Higgins, ³Department of Psychology, University of Arizona, Tucson, ⁴Escuela de Psicología, Universidad Católica del Norte, ⁵Laboratorio de Neurociencia Afectiva, Facultad de Psicología, Universidad del Desarrollo

Cat skin/fur odor exposure provides a reliable method to investigate neural circuits and mechanisms of defensive responses in rodents and may offer insight into mechanisms that mediate fear in humans. Here, we describe a protocol for investigating the role of the interoceptive cortex in threat responses in rats.

Neuroscience

A Visuospatial Planning Task Coupled with Eye-Tracker and Electroencephalogram Systems

Marcos Domic-Siede¹, Martín Irani², Joaquín Valdés³, María Rodríguez^4,5, Brice Follet^3,6, Marcela Perrone-Bertolotti^7,8, Tomás Ossandón³

¹Laboratorio de Neurociencia Cognitiva, Escuela de Psicología, Universidad Católica del Norte, ²Department of Psychology, University of Illinois Urbana-Champaign, ³Neurodynamics of Cognition Laboratory, Departamento de Psiquiatría, Pontificia Universidad Católica de Chile, ⁴Instituto de Ciencias de la Salud, Universidad de O’Higgins, ⁵Instituto de Ciencias de la Ingeniería, Universidad de O’Higgins, ⁶Department of Psychiatry, University Hospital of Saint-Etienne, ⁷Univ. Grenoble Alpes, Univ. Savoie Mont Blanc, CNRS, LPNC, ⁸Institut Universitaire de France (IUF)

The study of cognitive planning combining EEG and eye-tracking systems provides a multimodal approach to investigate the neural mechanisms that mediate cognitive control and goal-directed behavior in humans. Here, we describe a protocol for investigating the role of brain oscillations and eye movements in planning performance.

Neuroscience

Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor

Jeanette Wihan¹, Isabell Karnatz¹, Isabelle Sébastien¹, Ralf Kettenhofen¹, Benjamin Schmid², Christian Clausen², Benjamin Fischer¹, Rachel Steeg³, Heiko Zimmermann^1,4,5,6, Julia C. Neubauer^1,4

¹Fraunhofer Project Center for Stem Cell Process Engineering, Fraunhofer Institute for Biomedical Engineering IBMT, ²Bioneer A/S, ³Fraunhofer UK Research Ltd, Technology and Innovation Centre, ⁴Fraunhofer Institute for Biomedical Engineering IBMT, ⁵Department of Molecular and Cellular Biotechnology, Saarland University, ⁶Facultad de Ciencias del Mar, Universidad Católica del Norte

This article describes a protocol for the generation of human induced pluripotent stem cell-derived neurons in a benchtop 3D suspension bioreactor.