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Key Laboratory of Gastrointestinal Cancer (Fujian Medical University)

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Hansjörg Habisch^*1, Fangrong Zhang^*1,2, Qishun Zhou¹, Tobias Madl¹

¹Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Molecular Biology and Biochemistry, Medical University of Graz, ²Ministry of Education, School of Basic Medical Sciences, Key Laboratory of Gastrointestinal Cancer (Fujian Medical University)

The present protocol describes the preparation and quantitative measurement of free and protein-bound arginine and methyl-arginines by ¹H-NMR spectroscopy.