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Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province

1 ARTICLES PUBLISHED IN JoVE

Medicine

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

Chengchen Huang^1,2, Jiahe Han^1,2, Shuo Sun^1,2, Guosheng Fu^*1,2, Min Shang^*1,2

¹Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, ²Department of Cardiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University Hangzhou

Here, we describe a protocol to isolate a sufficient amount of single non-cardiomyocytes with high viability from a post-myocardial infarction (MI) mouse heart. This can be used for subsequent single-cell sequencing, flow cytometry analysis, and primary cell culture.