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Schepens Eye Research Institute of Mass. Eye and Ear

1 ARTICLES PUBLISHED IN JoVE

Biology

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Tessa C. Fitch^*1,2, Scott I. Frank^*1, Yutong Kelly Li¹, Magali Saint-Geniez^1,2, Leo A. Kim^1,2, Daisy Y. Shu^1,2

¹Schepens Eye Research Institute of Mass. Eye and Ear, ²Department of Ophthalmology, Harvard Medical School

The metabolic status of human retinal pigment epithelial cells (H-RPE) reflects their health and function. Presented here is an optimized protocol for examining the real-time metabolic flux of H-RPE using high-resolution respirometry.