Vlaams Instituut voor Biotechnologie (VIB)
3 ARTICLES PUBLISHED IN JoVE
Neuroscience
In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration
Eveliina Pollari 1,2, Robert Prior 1,2, Wim Robberecht 1,2,3, Philip Van Damme 1,2,3, Ludo Van Den Bosch 1,2
1Department of Neurosciences, Experimental Neurology, KU Leuven - University of Leuven, 2Center for Brain & Disease Research, Laboratory of Neurobiology, VIB, 3Department of Neurology, University Hospitals Leuven
The measurement of nerve conduction is a useful tool to assess mouse models of neurodegeneration but it is frequently only applied to stimulate the sciatic nerve in hindlimbs. Here, we describe a technique to measure compound muscle action potential (CMAP) in vivo in the mouse forelimb muscles innervated by the brachial plexus.
Neuroscience
Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
Katarina Stoklund Dittlau 1,2, Emily N. Krasnow 1,2, Laura Fumagalli 1,2, Tijs Vandoorne 1,2, Pieter Baatsen 3,4, Axelle Kerstens 3,4, Giorgia Giacomazzi 5, Benjamin Pavie 3,4, Elisabeth Rossaert 1,2, Jimmy Beckers 1,2, Maurilio Sampaolesi 5, Philip Van Damme 1,2,6, Ludo Van Den Bosch 1,2
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, 2VIB Bio Imaging Core, KU Leuven - University of Leuven, 3Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, 4Department of Neurology, University Hospitals Leuven
We describe a method to generate human motor units in commercially available microfluidic devices by co-culturing human induced pluripotent stem cell-derived motor neurons with human primary mesoangioblast-derived myotubes resulting in the formation of functionally active neuromuscular junctions.
Current Methods In ALS Research
Yvonne E. Klingl 1,2, Sandrine Da Cruz 3, Ludo Van Den Bosch 1,2
1Experimental Neurology and Leuven Brain Institute (LBI), Department of Neurosciences, KU Leuven - University of Leuven, 2Laboratory of Neurobiology, Center for Brain & Disease Research, Vlaams Instituut voor Biotechnologie (VIB), 3VIB-KU Leuven Center for Brain & Disease Research, Department of Neurosciences, KU Leuven, Leuven Brain Institute
Current Methods In ALS Research
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