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Delaware State University

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Karla R Sanchez¹, Mahlet D Mersha¹, Harbinder S Dhillon¹, Murali K Temburni¹

¹Department of Biological Sciences, Delaware State University

Exposure to environmental toxins can acutely impact developing embryos. Endocrine disrupting chemicals such as bisphenols are known to adversely affect the nervous system. Here we describe a protocol using an in vitro vertebrate (chick embryo) neuronal network model to study the functional impact of toxin exposure on early embryos.

Developmental Biology

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Mahlet D. Mersha¹, Karla R. Sanchez², Murali K. Temburni², Harbinder S. Dhillon²

¹Department of Neurology, Johns Hopkins School of Medicine, ²Department of Biological Sciences, Delaware State University

Exposure to environmental toxicants can acutely impact development with long-term effects. Detailed protocols are provided to illustrate a strategy using an effective lab model to study the effect of early embryonic exposure to bisphenol A. We provide fecundity and behavioral assays to monitor the effectiveness of our toxicant exposure bio-assays.